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absolute human mitochondrial dna copy number quantification qpcr assay kit  (ScienCell)

 
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    ScienCell absolute human mitochondrial dna copy number quantification qpcr assay kit
    Absolute Human Mitochondrial Dna Copy Number Quantification Qpcr Assay Kit, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/absolute+human+mitochondrial+dna+copy+number+quantification+qpcr+assay+kit/absolute+human+mitochondrial+dna+copy+number+quantification+qpcr+assay+kit/pm40593928-429-10-20
    Average 90 stars, based on 1 article reviews
    absolute human mitochondrial dna copy number quantification qpcr assay kit - by Bioz Stars, 2026-10
    90/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Tramadol suppresses growth of orthotopic liver tumors via promoting M1 macrophage polarization in the tumor microenvironment.
    Article Snippet: Tumor-associated macrophages (TAMs) are major infiltrating immune cells in liver cancer.. They are polarized to anti-tumor M1 type or tumor-supporting M2 type in a dynamic changing state.. Tramadol, a synthetic opioid, exhibits tumor-suppressing effect in several cancers, but whether it plays a role in TAMs polarization is uncertain.

    Article Title: Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo.
    Article Snippet: .. The Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (Sciencell, Cat#8948, CA, USA) was used to identify mtDNA copy number in target cells after mitochondria transplantation. .. The manufacturer’s reference genomic DNA sample was used as reference. qPCR was performed at following conditions: 95 ◦C for 10 min for initial denaturation, 32 cycles of 95 ◦C for 20 s, 52 ◦C for 20 s, and 72 ◦C for 45 s (Master Cycler, Eppendorf, Germany).

    Article Title: Fasudil and viscosity of gelatin promote hepatic differentiation by regulating organelles in human umbilical cord matrix-mesenchymal stem cells.
    Article Snippet: .. Mitochondrial DNA copy number estimation Mitochondrial DNA (mtDNA) copy number was determined using the Absolute Human Mitochondrial DNA Copy Number Quantification qPCR assay kit (ScienCell, CA, USA). .. Before assessing the mtDNA copy number, we isolated total DNA using the QIAamp DNA Mini Kit (Qiagen).

    Article Title: Fasudil and viscosity of gelatin promote hepatic differentiation by regulating organelles in human umbilical cord matrix-mesenchymal stem cells
    Article Snippet: .. Mitochondrial DNA (mtDNA) copy number was determined using the Absolute Human Mitochondrial DNA Copy Number Quantification qPCR assay kit (ScienCell, CA, USA). .. Before assessing the mtDNA copy number, we isolated total DNA using the QIAamp DNA Mini Kit (Qiagen).

    Article Title: Influence of Cellular Aging on Liver Stiffness in Patients With Hepatitis C Virus Achieving Sustained Viral Response
    Article Snippet: Relative telomere length (RTL) was measured in cfDNA using the Absolute Human Telomere Length Quantification quantitative polymerase chain reaction (qPCR) Assay Kit (AHTLQ; ScienCell Research Laboratories) according to the manufacturer's instructions. .. The mitochondrial copy number was measured in cfDNA using the Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (AHMQ; ScienCell Research Laboratories) according to the manufacturer's instructions. .. The following markers were assessed: parameters of inflammation were high-sensitivity C-reactive protein (hsCRP; Roche latex-enhanced immunoturbidimetric assay), interleukin 6 (IL-6; Diaclone), chemokine C-X-X ligand with motif 10 (CXCL10; Invitrogen), chemokine C-C ligand with motif 11 (CCL11; Sigma-Aldrich), and chitinase-3-like protein 1 (CHI3L1; Invitrogen); activation parameters were soluble CD14 (sCD14; FineTest) and soluble CD163 (sCD163; FineTest); oxidative stress parameter was isoprostanate-8 (Iso-8; Cayman Chemical); hemostasis parameter was von Willebrand Factor (vWF; Invitrogen); and parameter of physiological function was vitamin D (MyBioSource).

    Article Title: Temporal analysis of doxorubicin-induced cardiac toxicity and hypertrophy.
    Article Snippet: Mitochondrial DNA copy number assessment Total DNA was extracted using the QIAamp DNA Mini Kit (QIAGEN, Germany). .. The mitochondrial DNA copy number (mtDNA-CN) was quantified with the Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (ScienCell, USA). .. Real-time PCR (qPCR) npj Systems Biology and Applications | (2025) 11:67 16 was performed on a CFX Connect Real-Time PCR Detection System (Bio-Rad, UK).

    Article Title: Long-Term Photobiomodulation of Cellular and Mitochondrial Activities in IMR-32 Cells Using an 810 nm Light-Emitting Diode
    Article Snippet: In photobiomodulation (PBM), biological tissues are stimulated using low-energy light.. Mitochondria are thought to be integral to the process, and PBM effects vary with light parameters.. Biphasic curves are commonly used to describe the dose response of PBM but do not fit in some cases.

    Article Title: Mitochondrial Dysfunction Involved in the Cytotoxicity of Tramadol in Human Endometrial Carcinoma Cells.
    Article Snippet: .. The quantification of mtDNA copy number was assessed by the Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit. (ScienCell Research Laboratories, San Diego, CA, USA) according to the manufacturer’s protocol. .. Briefly, after the tramadol treatment, genomic DNA extraction from cells using the gSYNCTM DNA Extraction Kit (Geneaid, Taipei, Taiwan).

    Transplantation Assay:

    Article Title: Mitochondrial transplantation: Effects on chemotherapy in prostate and ovarian cancer cells in vitro and in vivo.
    Article Snippet: .. The Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (Sciencell, Cat#8948, CA, USA) was used to identify mtDNA copy number in target cells after mitochondria transplantation. .. The manufacturer’s reference genomic DNA sample was used as reference. qPCR was performed at following conditions: 95 ◦C for 10 min for initial denaturation, 32 cycles of 95 ◦C for 20 s, 52 ◦C for 20 s, and 72 ◦C for 45 s (Master Cycler, Eppendorf, Germany).



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    Longitudinal analysis of mtDNA copy numbers in the supernatants of activated naïve CD8 + T cells isolated from 6 older adults Comparison by two-tailed unpaired t-test. ∗∗∗∗ P < 0.0001.

    Journal: STAR Protocols

    Article Title: Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8 + T cells

    doi: 10.1016/j.xpro.2025.103612

    Figure Lengend Snippet: Longitudinal analysis of mtDNA copy numbers in the supernatants of activated naïve CD8 + T cells isolated from 6 older adults Comparison by two-tailed unpaired t-test. ∗∗∗∗ P < 0.0001.

    Article Snippet: Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (AHMQ) , ScienCell , Cat# 8948.

    Techniques: Isolation, Comparison, Two Tailed Test

    Preparation of master mix for reference sample

    Journal: STAR Protocols

    Article Title: Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8 + T cells

    doi: 10.1016/j.xpro.2025.103612

    Figure Lengend Snippet: Preparation of master mix for reference sample

    Article Snippet: Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (AHMQ) , ScienCell , Cat# 8948.

    Techniques:

    Preparation of master mix for target sample

    Journal: STAR Protocols

    Article Title: Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8 + T cells

    doi: 10.1016/j.xpro.2025.103612

    Figure Lengend Snippet: Preparation of master mix for target sample

    Article Snippet: Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (AHMQ) , ScienCell , Cat# 8948.

    Techniques:

    Absolute copy numbers of mtDNA released by activated naïve CD8 + T cells derived from 856 healthy adults and 665 lung adenocarcinoma patients Comparison by two-tailed unpaired t-test. ∗∗∗∗ P < 0.0001; NS, not significant.

    Journal: STAR Protocols

    Article Title: Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8 + T cells

    doi: 10.1016/j.xpro.2025.103612

    Figure Lengend Snippet: Absolute copy numbers of mtDNA released by activated naïve CD8 + T cells derived from 856 healthy adults and 665 lung adenocarcinoma patients Comparison by two-tailed unpaired t-test. ∗∗∗∗ P < 0.0001; NS, not significant.

    Article Snippet: Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (AHMQ) , ScienCell , Cat# 8948.

    Techniques: Derivative Assay, Comparison, Two Tailed Test

    Journal: STAR Protocols

    Article Title: Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8 + T cells

    doi: 10.1016/j.xpro.2025.103612

    Figure Lengend Snippet:

    Article Snippet: Absolute Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (AHMQ) , ScienCell , Cat# 8948.

    Techniques: Recombinant, Saline, Red Blood Cell Lysis, Cell Isolation, Software, Cell Culture